Skin

Human Living Skin Equivalent

Epistem's in vitro 3D Human Living Skin Equivalent model is highly reproducible and closely resembles fully stratified human epidermis. Cultured primary human keratinocyte and fibroblast cells make this industry standard model ideal for studying the effects of novel therapeutic agents on the epidermis. Donor skin used to isolate cells can be specified to ensure the model is representative of the required skin type (ethnicity, age, body site etc.).

Epistem's team have many years of experience culturing Living Skin Equivalents, ensuring reproducible and high quality data which can be used for claims and patent support. This service is supported by Epistem's fully GCLP accredited histology and immunohistochemistry service.

Human Living Skin Equivalent Model Description

Freshly isolated human keratinocyte and fibroblast cells are cultured within a collagen gel at the air-liquid interface for up to 10 days. Novel therapeutic agents can be delivered topically and systemically via the culture media.

Cell types

  • Keratinocytes
  • Fibroblasts
  • Option to incorporate melanocytes

Readouts

  • Histometric analysis
  • Proliferation, differentiation and apoptotic markers
  • WST-1 assay for cytotoxicity screening
  • UV damage marker quantification
  • Protein expression
  • Gene expression including anti-ageing and anti-oxidant pathways

Further readouts available on request.

H&E staining of human living skin equivalent

H&E staining of human living skin equivalent

Fully-differentiated Human Living Skin Equivalent model

Fully-differentiated Human Living Skin Equivalent model

© 2013-17 Epistem Ltd. : : last modified: 04 Nov 2013

Epistem Ltd is a division of genedrive plc