Inflammation

Adoptive T-Cell Transfer Model

Epistem offers an adoptive T-cell transfer-mediated model of chronic colitis. This model is dependent on adaptive immunity and is suitable for the evaluation of therapies that can be administered either prophylactically or therapeutically.

Adoptive T-Cell Transfer Model Description

Colitis is induced by transferring naïve CD4+CD62L+ T-cells from donors to recipients. Disease results from the activation of donor T-cells by luminal antigens. This adoptive T-cell transfer model typically runs over 4-5 weeks. Colitis is characterised by discontinuous lesions in the large bowel, erosion and occasional ulceration; affected areas demonstrate significant infiltration into the mucosa and submucosa.

Primary Readouts

  • Large bowel histopathology

Further Readouts

  • Large bowel weight : length ratio
  • Immunohistochemistry and immunofluorescence
  • Physiological
  • Diarrhoea severity
  • Weight loss
  • FACS analysis of inflammatory cell response
  • Multiplex analysis of serum and tissue cytokines
  • Protein expression
  • Gene expression
  • Haematological analysis

H&E staining of normal colon

H&E staining of normal colon

H&E staining of diseased colon following CD4+CD62L+ T-cell transfer

H&E staining of diseased colon following CD4+CD62L+ T-cell transfer

© 2013-17 Epistem Ltd. : : last modified: 14 Mar 2014

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