GI Toxicity and Mucositis
3D Intestinal Organoid Model
Epistem offers a 3D predictive in vitro model of epithelial regeneration using primary intestinal crypt organoids. This well established model can be used for determining the efficacy and toxicity of novel therapeutic agents on normal intestinal tissue.
The intestinal crypts are cultured in conditions designed to mimic the stem cell niche and allow proliferation and differentiation to occur. All differentiated cell types are present and the epithelial cell hierarchy closely resembles that observed in vivo. The intestinal crypt organoids can be perpetuated indefinitely by weekly passaging. Epistem’s organoid model is particularly useful for studying the regulation of intestinal stem cell self-renewal and differentiation. In addition, a proprietary Primequant® model is also available and both models can be used to predict the in vivo response to novel therapeutic agents.
Multiple therapeutic agents can be quickly and efficiently screened for proliferative or cytotoxic effects. Screening requires only small volumes of novel therapeutic agent and is possible where in vivo screening is infeasible.
Intestinal Organoid Model Description
Intestinal crypts are isolated and maintained in tissue culture allowing organoids to establish. The effect of a novel therapeutic agent on organoid viability and production of crypt-like structures is scored on day 4 or 5. Multiple therapeutic agents can be assessed using a 96-well plate format.
- Cell number
- Extent of branching
- Changes in proliferation and differentiation
- Protein expression of target pathways
- Gene expression of target pathways