Biodefense

Acute Radiation Syndrome Models

Epistem provides models of Gastrointestinal (GI-ARS) and Haematopoietic (H-ARS) Acute Radiation Syndrome and Delayed Effects of Acute Radiation Exposure (DEARE). These models can be used to provide mechanism of action data and can include full histological evaluation of the irradiated gastrointestinal epithelium. Epistem's models of ARS have been used by NIAID to evaluate potential mitigators of radiation exposure for over 5 years.

Acute Radiation Syndrome

X-ray irradiation is performed with bone marrow shielding where required. Full dosimetry of the 320kV source is also performed for each study run.

Gastrointestinal - Acute Radiation Syndrome

Radiation kills the rapidly proliferating clonogenic cells in intestinal crypts. If all the clonogenic cells are killed, the crypt will die. If sufficient crypts die, ulcers will form, resulting in diarrhoea, infection, dehydration and death.

Readouts

  • Survival
  • Diarrhoea duration and severity
  • Crypt Survival
  • Apoptosis and proliferation
  • Cytokine analysis by multiplex
  • Protein expression
  • Gene expression
  • Histology

Haematopoietic - Acute Radiation Syndrome

The effects of H-ARS manifest later than GI-ARS, however, the bone marrow is highly sensitive. A lower dose of irradiation is therefore required and detrimental effects manifest over a longer time course than GI-ARS (apparent from day 10 onwards).

Readouts

  • Survival
  • Histology of the bone marrow
  • Blood counts
  • Cytokine analysis by multiplex
  • Protein expression
  • Gene expression

Intestine 4 days post cytotoxic insult showing a completely denuded mucosa

Intestine 4 days post cytotoxic insult showing a completely denuded mucosa

Intestine 6 days post irradiation showing regenerating epithelium

Intestine 6 days post irradiation showing regenerating epithelium

© 2013-17 Epistem Ltd. : : last modified: 19 Nov 2013

Epistem Ltd is a division of genedrive plc